Does anyone have suggestion on how to sterilise high Mw polyethylene oxide (PEO) to be used for cell cultures?

I am trying to sterilise high molecular weight polyethylene oxide (~400,000 Da) in DMEM for culturing bmMSCs. I have done online search for relevant information, but most studies were using PEG...

25 November 2020 3,134 8 View

GC 7890; MS 7000C what are some possibilities of dramatic decreased in sensitivity if there are no leaks, and the source is recently cleaned?

11 April 2016 7,943 4 View

Cooling module for CHEF Mapper XA just broke... Can CHEF mappers be run in 4C cold rooms?

Normally, I have 0.5 x TBE buffer running at 14C, and the cooling module keeps the buffer at this temperature. Ours seems to be broken, since it is not making the clicking noise it usually does,...

04 February 2016 217 1 View

Why does my bacteria culture smells funky?

I have been doing large scale expression of this protein of interest name, yqjD. Previously was fine, did a couple times, got some proteins and did several crystal screenings. Now that the...

10 November 2015 1,139 9 View

Are there any advantages miRNA precursor when compared with miRNA mimic?

13 June 2015 8,787 6 View

Does anyone use the CRISPR-Cas9 knockout plasmids from Santa Cruz?

25 February 2015 3,363 45 View

Is it possible to get cyclic voltammetry of activated carbon in 5 M KOH scanned from -1 V to 1 V?

The electrochemical setup is two-electrode system with activated carbon as anode and cathode?

22 September 2014 4,155 12 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View