Is there anyone who knows about a protocol for latex coated antibody for turbidimetric assay?

07 November 2015 1 358 Report

i'm looking for a protocol to to coate latex to antibody for measuring latex-antibody-antigene complex with turbidimetry

Andrew Kirkham

The easiest thing to do would be to buy a latex kit and follow the protocol that comes with it. For a covalent attachment, EDAC would be a good place to start. If you are going to use passive absorption, there are a great number of protocols to be found online, but in essence it is a matter of mixing the antibody with the latex in a suitable buffer, and using something like BSA as a blocker.

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