My Sp1K since their purchase at passage 8 have been very slow in growing, but now they stopped. We used all medium and reagents as raccomended, they are Mycoplasma free, but they are stopped from about 10 days, now they are at passage 13 is senescence possible already in this cell line?
Dear Willems Daniela,
I used condition medium (as supplement) for such situation. Quality of falcons or plates is general factor too,
Good luck!
Thank you Liubov Yurjevna Vergun,
I'll follow your suggestion and hope it works !!!!
Hi Willems, I don't have experience with this line but what you describe is the classical response of a cell line to an FBS that they do not like (they start growing but gradually begin to stop over time).
Cell lines (generally) do not become senescent as primary cells do so this is unlikely (especially in such a short time scale and without the "classic" senescent phenotype such as increase in cell size, "fried egg" phenotype etc).
I am guessing that you did not purchase FBS from the supplier of the cells and that you are using the FBS you already had "in-lab"?
I would purchase a small (100 ml) amount of a good quality FBS (such as from Gibco or ATCC) and try to grow some of the cells in this to see if it makes a difference (as mentioned above supplementation with some conditioned media may also help).
Also it is worth logging the problem with the supplier of the line (they may give you more cells or point out where you are going wrong).
Sorry I can't be more help,
Gary
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