Is there anyone who can provide me ovarian cancer NuTu-19 cell line?

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Does any of you have information on CO2 absorption coefficients in infrared spectrum at 4.2 um ?

I am looking to built a CO2 sensor based on near infrared (4.2 um) absorption of the gas. For the calculations, it would be handy to know the absorption coefficient, only, this seems difficult to...

11 December 2021 5,545 3 View

Will Oil Prices Ever Recover back to $65/bl ?

Oil prices have waxed and waned following COVID-19, but supply pressures and compliance from OPEC member countries have provided a much-needed balance int he last decade. With the U.S. becoming...

09 October 2020 630 2 View

What is the best lysis buffer for homogenization of tissue samples to protein carbonyl content ELISA?

Hi. I have a commercially available, widely used, ELISA kit for testing protein carbonyl content. This is from CellBioLabs. I'm wondering what lysis buffers others have used when doing homogenates...

12 July 2020 7,330 0 View

How to calculate spatial autocorrelation with ordinal data (using R)?

I'm working on a data set consisting of data about geographical districts. Some of the variables are continuous for which I manage to calculate global and local autocorrelation using R package...

05 April 2020 4,787 0 View

How to differentiate a zircon core resulting from leaching by metamorphic fluids or melt from one whose surface results from detrital rounding?

Textural analysis of zircons is the first step of study that allows selection of targets for geochemical and isotope analysis (Hf isotopes, REE, 18O, etc.), which are necessary to understand...

09 March 2020 367 5 View

Is wireless key agreement for finite key sizes provably secure?

Background: Alice and Bob collect N wireless channel measurements, quantize secret bits out of them, correct for errors and perform privacy amplification to reduce the eavesdropper information...

03 September 2019 8,364 3 View

How can I introduce a new institution in Research Gate?

How can I introduce a new institution?

07 March 2019 6,178 9 View

Trouble with Cell Trace Violet for MLR?

Hello, I m currently doing MLR with human PBMC and I m using CTV in order to observe the proliferation. The two first experiences worked well with a niveau proliferation with CTV. But since then...

12 December 2018 2,598 3 View

Database for primary commodity exports by country since 1960?

Dear fellow researcher, I am currently working on a study researching the economic causes of civil war. As stated in previous studies of this topic (Collier&Hoeffler, Fearon, Humphreys etc.),...

29 November 2018 5,207 1 View

What kind of coupling agent should I use to improve the interface between PLA and glass fibers ?

Do I need to use a coupling agent when mixing PLAwith short glass fibers ? If yes, hat kind of coupling agent should I use to improve the interface between PLA and glass fibers ? Thanks

12 November 2018 2,833 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View