There seem to be different subtypes of Lipofectamine available with perhaps different effectiveness on different cell types and conditions. But sometimes, one may need to repeat or optimize many conditions in an experiment, especially with difficult cells.

I ve miserably failed with LIPO -LTX in regard with transfection to astrocytes ,as size of the construct increases. With few Kb construct size also there was poor transformant efficiency .But the same case differs with easily transfectable cells like HEK.

Hi Aifang,

for your cells, I recommend NeuroMag transfection reagent, here you can find the information and look at the citation databsd you will see many publications

http://www.ozbiosciences.com/transfection-cell-specific/52-neuromag-neuron-transfection-magnetofection.html

here you can see several publications using this transfection reagent for CGN

http://www.ncbi.nlm.nih.gov/pubmed/18852027

http://www.ncbi.nlm.nih.gov/pubmed/20447456

Good Luck

Olivier

Thanks Olivier, it helps a lot!