In the case of sample shortage, usually biopsy, you have to use whole sample for RNA extraction but in the single cell transcriptome analysis you use just pick up one cell and make cDNA. Then finally microarray or sequencing is performed.
High-throughput techniques gives us a list of differentially or co-expressed genes. Now, which methods can infer a pathway from a list of genes (static or dynamic pathways)? How do they work?
08 September 2013 5,770 4 View
In systems biology, most of the time its needed to integrate several layers of information (e.g. genomics, proteomics and transcriptomics). There is software in some levels for example...
07 August 2013 2,358 19 View
Chronic lung diseases are complicated. In most of the chronic cases, tissue remodeling is observed. Inflammation and fibrosis are two arms of this phenomenon. In some diseases fibrosis is dominant...
31 December 2012 2,498 6 View
I wonder if someone know how to isolate fibroblast cells directly from a tissue without using cell culture techniques. A 1mm3 biopsy may contains very limited number of fibroblast cells. Is there...
11 December 2012 9,033 28 View
I have studied a lot about oxidative stress in COPD and asthma and have heard a bit about its induction during environmental factors. For e.g. the heat of cold induces it. Actually, I don't know...
11 December 2012 7,053 10 View
In the case of sample shortage, usually biopsy, you have to use whole sample for RNA extraction but in the single cell transcriptome analysis you use just pick up one cell and make cDNA. Then...
02 March 2012 9,665 5 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
23 February 2021 3,840 3 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View
Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...
21 February 2021 8,945 3 View
I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...
20 February 2021 6,879 3 View