PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

What is the correct way to apply a Design effect for sample size calculation when the sampling method is linear systematic random sampling?

When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?

20 January 2021 5,328 3 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

Handheld Portable Raman spectroscopy systems - recommendations and experience?

Hi all, I am looking for some general feedback and information from those that have experience using handheld/portable systems? I am looking at the prospects of using an off the shelf system from...

07 February 2021 9,691 3 View

Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

What could be the reason for a broad peak of ADC(Antibody Drug Conjugates) using HIC-UV method (Hydrophobic Interaction Chromatography)?

Hi, We are working on DAR (Drug to Antibody Ratio) estimation of ADC using the following HIC-UV method. For some of the unconjugated mab we getting a sharp peak but for our desired mab we are...

15 November 2020 7,359 0 View

Could anyone professional help me to solve this problem?

I have installed the software BRUKER Flexanalysis 3.4 on my computer successfully. But when I tried to import the MS data produced by BRUKER MALDI-TOF-MS, I met with an error described in the...

10 November 2020 8,205 1 View

How to set up the parameters on an NMR instrument to run deuterated sulfuric acid as a solvent?

I have a compound which is only stable in concentrated sulfuric acid and I'd like to get a NMR spectrum so I can deduce the structure. I use a 300 MHz Bruker instrument and the software is topspin.

25 September 2020 5,218 3 View

Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability?

I have a question for KLH experts. Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability? Is a T- cell expressing KHL- or...

24 September 2020 2,931 1 View

How can I improve the rietveld refinement model and lower the R bragg factor and chi-square?

I measured a fluconazole drug sample and found 2 phases. Then, for the first time, I tried to apply the rietveld refinement method using Match! and got relatively high values for both R bragg...

27 July 2020 9,492 4 View

The shoulder on the XRD peak. What could it be?

Dear RG community! I am obtaining very weird peaks on the XRD pattern. There is a shoulder on the left side of the peak looking like a step. Arrow on the picture. It is about 2-3% of the Ka1 peak...

25 June 2020 8,500 12 View

Quantifying ADC values from a ROI?

I currently have both DWI and ADC sequences however I want to calculate the mean ADC value for a defined ROI (ischemic stroke lesion). I currently use ITK-SNAP, Image J and Horos for viewing...

03 March 2020 9,749 4 View

What is the optimal way to read the voltage of each individual cell when the cells are connected in series by integrating each cell with L-bridge?

Hi there, My prototype is about a battery energy storage system. I am seeking to achieve a state of charge balancing among the cells by controlling and monitoring each individual cell. The cells...

22 February 2020 7,102 4 View