How can I dissolve bovine fibrinogen?

09 October 2014 6,742 16 View

What are the pros and cons of performing immunohistochemical study and qPCR?

Which one will be provide more reliable results and minimal errors during procedure? I need to perform either of these to show there is increased protein/mRNA expression because of scaffold...

11 December 2013 1,530 1 View

What is the effect of Ferridex, a FDA approved MRI contrast agent which is Iron oxide nanoparticles(NPs), on tumors?

What do you think these nanoparticles dissolute into ferrous ions? I have read that cancer tissue requires iron for its proliferation, so are we feeding tumors with this iron oxide NPs?

10 November 2012 2,596 16 View

For drug delivery to brain, what are the routes of administration? (like intracarotid etc). What are their advantages and problems?

Please elaborate your answer

08 September 2012 9,198 5 View

What do you think about the role of cytomegalovirus (CMV) role in glioma?

There are many reports stating the presence of CMV in glioma patients. Does it play any role in glioma? Does it cause any problem in glioma patients?

08 September 2012 7,784 12 View

I have difficulties in resuspending lyophilized nano-particles. What might be the problem?

Any insights would be appreciated.

08 September 2012 5,053 31 View

How do I measure a drug release profile for a drug encapsulated in a nanoparticle?

The problem is that the drug is unstable at pH 7.4.

08 September 2012 6,397 16 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View