Is there any way to measure a redox potential of a certain protein in vivo?

28 April 2014 1 6K Report

Say by screening the electron transport chain with reducing agents of different redox potential? Can you recommend what to read please?

Taras Pasternak

Hi, SErgeii, for in vivo you can try to use different GFP redox-sensitive markers. here are some references: http://www.freepatentsonline.com/7015310.html

www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1003902

For the isolated protein you can try electrophoretic redox Western blot method. . http://www.sciencedirect.com/science/article/pii/S0891584912011446

Good luck!

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