I want to assign the target of a specific micro RNA , Is there any valid protocol for Luciferase assay without using commercial kits?
Dear Milad
Please see the following file. Hope to be useful to you.
You might be interested in this thread:
https://www.researchgate.net/post/Lysis_buffer_for_luciferase_assays
you can see this paper:
Naderi S, Melchior DL. A firefly luciferase assay for subnanomolar concentrations of amphipathic substances. Analytical biochemistry. 1990 Nov 1;190(2):304-8.
Best
thank you all .
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