Is there any user friendly protein modelling software rather than MODELLER?

Abhijit Chakraborty @Abhijit_Chakraborty8

01 January 2016 0 5K Report

For using homology or comparative modeling of protein three-dimensional structures

Badges
Science topic

More Abhijit Chakraborty's questions See All

CAG promoter vs CMV promoter which one is better for high expression of protein?

I am constructing one plasmid vector for mammalian expression. I am going to use it for Purification of Protein for structural analysis. Which Promotor is the best one and can give me high yield?

07 August 2018 779 1 View

Which is the best antibody to use in immunoprecipitation (IP) as well as Western Blotting for the study of BRCA1 and BRCA2?

I need to do IP as well as Western Blotting for the endogenous and overexpression BRCA1 and BRCA2 protein. Which is the best one to use?

31 December 2016 6,370 3 View

How can I confirm that a sequence variation is a mutation or Polymorphism?

I found a new variant (a substitution) in exon 20 of BRCA1 gene. This is not reported in HGVS, BIC or any other mutation database. The variant was found after sequencing with both the forward and...

03 April 2016 4,231 14 View

Modeller 9.12 shows the following result. How can this be rectified? (File attached)

During protein modelling, Modeller 9.12 shows some problem (see attached file). How can I minimize the error?

05 June 2013 5,035 2 View

How do I calculate the annealing temperature of my PCR reaction?

Tm value of one of my primer is 52.8 (50mM NaCl) and another one is 54.5 (50mM NaCl) as given by the primer making company. Primer Sequence is – i> 5' TGT GTA TCA TAG ATT GAT GCT TTT GA 3' and...

05 June 2013 7,539 49 View

How can I re-concentrate my protein after gel-filtration Chromatography?

I isolated my protein complex from mammalian cells. Purify it using affinity purification methods. but after running chromatography the concentration became very low to go further structural...

01 January 1970 5,267 14 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View