Abhijit Chakraborty

7 Questions 10 Answers 0 Followers

Questions related from Abhijit Chakraborty

CAG promoter vs CMV promoter which one is better for high expression of protein?

I am constructing one plasmid vector for mammalian expression. I am going to use it for Purification of Protein for structural analysis. Which Promotor is the best one and can give me high yield?

08 August 2018 753 1 View

Which is the best antibody to use in immunoprecipitation (IP) as well as Western Blotting for the study of BRCA1 and BRCA2?

I need to do IP as well as Western Blotting for the endogenous and overexpression BRCA1 and BRCA2 protein. Which is the best one to use?

01 January 2017 6,349 3 View

How can I confirm that a sequence variation is a mutation or Polymorphism?

I found a new variant (a substitution) in exon 20 of BRCA1 gene. This is not reported in HGVS, BIC or any other mutation database. The variant was found after sequencing with both the forward and...

04 April 2016 4,208 14 View

Is there any user friendly protein modelling software rather than MODELLER?

For using homology or comparative modeling of protein three-dimensional structures

01 January 2016 5,212 0 View

Modeller 9.12 shows the following result. How can this be rectified? (File attached)

During protein modelling, Modeller 9.12 shows some problem (see attached file). How can I minimize the error?

06 June 2013 5,013 2 View

How do I calculate the annealing temperature of my PCR reaction?

Tm value of one of my primer is 52.8 (50mM NaCl) and another one is 54.5 (50mM NaCl) as given by the primer making company. Primer Sequence is – i> 5' TGT GTA TCA TAG ATT GAT GCT TTT GA 3' and...

06 June 2013 7,506 49 View

How can I re-concentrate my protein after gel-filtration Chromatography?

I isolated my protein complex from mammalian cells. Purify it using affinity purification methods. but after running chromatography the concentration became very low to go further structural...

01 January 1970 5,235 14 View