I added benzalkonium chloride to the water in the pan of co2 incubator but the condition of cells is deteriorating. can it be because of benzalkonium chloride?
Dear Himani,
BAC is normally a mixture of compounds with different side chains (C8 to C18). The C8 one has a boiling point of 100°C (http://www.chemicalbook.com/ChemicalProductProperty_US_CB1712971.aspx). Thus it is not unlikley that it evaporates to some extend. From literature and our cytotox studies I know the quartanary ammonium compounds are quite toxic.
Cheers
Stefan
It depend in which concentration that you added. Benzalkonium chloride (from Sigma Aldrich) can be added to the tray at the specified concentration (1:64 from a 10% solution or 1% working solution diluted as 1:50 to 1: 100, with no effect to the cells.
Dear Himani, please check out information on substances registered according to REACh at ECHA. http://www.echa.europa.eu/web/guest/information-on-chemicals/registered-substances
The (U.S.) National Library of Medicine's Hazardous Substances Data Bank (HSDB) includes a record for Benzalkonium Chloride Compounds. You can access this record via the web link I have provided. If you go to the "Animal Toxicity Studies" section of this record, there is a series of "/ALTERNATIVE and IN VITRO TESTS/ " studies of possible interest to you, e.g., " Benzalkonium Chloride concentrations of 0.01 and 0.02% did not result in growth retardation of cardiac fibroblasts that was significantly different from controls. However, concentrations of 0.033 and 0.10% resulted in significant retardation. The greatest degree of growth retardation of cardiac fibroblasts was achieved with a Benzalkonium Chloride concentration of 0.1%." (Disclaimer: Opinions are my own and are not necessarily aligned with those of NIH/NLM.)
http://toxnet.nlm.nih.gov/cgi-bin/sis/search2/r?dbs+hsdb:@term+@DOCNO+234
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