I have a mouse hippocampus that I need to preserve for a period of time. I ordered RNA later, but I read that also Ammonium sulfate buffer in certain concentration (with EDTA and sodium citrate) is a good preservative substituent of RNA later.
To my knowledge, the most of RNAse activity from fresh harvested tissue comes from tissue itself. I have very good experience with shock freezing mouse hippocampi in liquid nitrogen, and then extracting RNA by using standard Trizol procedure. This should give you values around 300-400ng of RNA on nanodrop for a mouse hippocampus
RNA is later immediately converted to cDNA for rtPCR and the dCt values I get for bActin are 15-16 with 100% primer efficiency confirming high yield RNA extraction.
The procedure of tissue harvesting and biomaterial preservation will also highly depend on what do you need from that hippocampus of course.
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