I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

How to measure porosity/diameter of electrospun nanofibers using imagej?

Hi all, I have SEM images of electrospun nanofibers and I'd like to measure its porosity/diameter. However I'm not sure how to do it with ImageJ or any other software. Can anybody help me please?...

27 July 2020 5,925 7 View

How important is it to check the psychometric properties of translated version of a gold standard scale?

There is a scale in English that has well established psychometric properties. I want to use the translated version of the scale in my study. Do I have to test all the psychometric properties of...

11 June 2020 3,951 14 View

Should solar irradiance be considered in Wh or kWh when designing a PV system in kW?

Hi, I am designing a PV system in kW and having a small confusion that should I be considering the solar irradiance in Wh or kWh in the PV calculations? Thank you.

02 July 2019 4,127 3 View

Can anyone provide a reliable protocol with tips and tricks for miRNA isolation from human plasma using Trizol?

Trizol protocol I am using, provides good total RNA concentration and quality but the miRNA spike (Cel-miR39) is not being amplified in qPCR. Starting volume: 600microlitre Plasma Elution:...

13 June 2019 2,228 9 View

Can anyone help me understanding why am I getting jagged amplification curve for my miRNAs in plasma sample?

I have used mircury exosome serum/plasma kit along with trizol for extraction of my miRNAs from human plasma. RNA concentration was 623.8ng on nanodrop with 260/280 ratio of 2.12. For RT-PCR and...

11 June 2019 3,679 4 View

What is the best way to purify the ear clip genomic DNA from TaqMan DNA extraction reagent?

13 October 2018 3,427 3 View

Significance of pAkt/Akt and pErk/Erk ratio?

Hi, I'm testing the effect of a drug on cardiomyocytes. The treated cells are then used for Akt and Erk ELISA. Can anyone tell me how do I interpret the pAkt/Akt or pErk/Erk ratio? For...

10 September 2018 3,959 3 View

Insulin concentration in culture medium?

Hi all, to mimic the hyperinsulinemia, what concentration of insulin should be used in the culture medium of growing cell lines (for example L6/MIN6) ? Also what should be the normal concentration...

21 March 2018 2,255 2 View

How to find quantitative determination of fe2+,fe3+ ratio from xps?

In my sample fe deped BaTiO3, both Fe2+ ferrous and fe3+ Ferric ion i.e.,multi valence states present which identified from XPS. How to find fe2+:Fe3+ ration present in my sample? which method I...

20 May 2017 8,210 4 View

Are there any thermochromic materials that reflect infrared light (~9000nm) at 10C and transmit infrared light at 25C?

So I know some metal-insulators (ex VO2) have thermochromic infrared-reflective properties, but most of these are insulators at low temperatures and conductive at high temperatures. Some...

07 February 2021 1,770 3 View

What is the role of top counter electrode when using perovskite thin-films in photoelectrochemical application?

I am currently working on perovskite based photoelectrodes, but I do not want to replace the counter electrodes including Ag, Au, will it have serious issue in the PEC performance?

13 January 2021 3,408 2 View

How to Interpret Photoluminescence Spectrum of Mixed Pb-Sn FA0.7MA0.3Pb0.7Sn0.3I3 Perovskites?

Dear Researchers, I hope you all will be in good health. I am writing this to request for your guidance. I am working on the fabrication of low bandgap Pb-Sn mixed perovskite with exact...

23 December 2020 1,147 1 View

How to know if Zinc Oxide is coated on the glass substrates?

Hello all I have prepared Zinc Oxide nanoparticles using sol gel synthesis. I tried to coat the ITO and glass substrates with the ZnO nanoparticles ( dispersed in ethanol). But I am not sure if...

08 December 2020 3,692 8 View

Is there any Perovskite validation App available?

Is the any App by which I can check if different elements of choice can form Perovskites. I mean any App or even Excel addin that can calculate the tolerance factor.

08 November 2020 9,849 2 View

Which PEIE to use?

Hello all Thanks for answering my previous questions. I am making perovskite LED and intend to use PEIE on zinc oxide layer as ETL. But I am not sure what version of PEIE to us. There is the one...

08 November 2020 5,710 3 View

Is there any possible stabilize metal-doped blue-emitting light perovskite through self-assembly method?

Now, I have got a metal-doped perovskite in which blue shift happens result. I tried to make it a more blue shift to get a stable and very blue perovskite. So I think is there any possible...

04 November 2020 8,384 3 View

Why perovskite thin film PLQY much higher than dispersion PLQY?

I doped Mn into perovskite. I found when up to the best doping amount, the thin film PLQY much higher than the dispersion PLQY in my experiment. I don't know what caused it, is there any theory...

04 November 2020 2,477 3 View

Solar cell Nyquist plots?

Hi, I study perovskite. Through the project, I studied the OPV cell, and the nyquist plot was obtained through impedance measurement. In the case of perovskite, the nyquist plot has two...

03 November 2020 1,973 2 View

Zno NPs seperation?

Thanks a lot for your answers. I have one more question: after synthesizing ZnO nanoparticles , I am not able to separate from the original solution (dispersion actually). The recipe is as...

23 October 2020 9,857 8 View