15 January 2018 5 4K Report

Protocol for strawberry tissue culture.

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Problem with beta galactosidase staining assay: X-gal crystals in staining solution?

Hello, I'm conducting a beta galactosidase staining assay for my fibroblasts using X-Gal. I dissolve X-Gal (stock 50mg/ml prepared in DMSO) in the staining buffer at a final concentration of...

25 March 2024 8,799 3 View

If OIBs are uncommon during Archean due to higher melting degrees, why Carbonatites are found during Archean?

The absence of Ocean Island Basalts (OIBs) in the Archean rock records is commonly attributed to the higher melting degrees associated with mantle plumes. However, this explanation raises...

14 March 2024 2,195 1 View

What is the best method for endophytic bacterial isolation?

Mostly bacteria living inside plant roots.

14 March 2024 4,602 0 View

Counterpoise (BSSE) Correction for more than 2 fragments?

What is the correct way to calculate the BSSE correction for a molecule having 3 fragments? Please see the attached image for details. I know the values are pretty close but still I would love to...

25 October 2023 3,990 1 View

Can I use TRIX index for coastal lagoon using DIN and PO4 instead of TN and TP?

Can I use DIN and PO4 in the given formula : The trophic index (TRIX) used in the present work has been given by the following formulation (Vollenweideret al.1998): TRIX = [log(Chla∙ (D%)O2 ∙ TN ∙...

26 September 2023 432 1 View

What was the mode of weathering/erosion during Hadean to Mesoarchean?

Many think that, because of the vastness of the oceans, the continents were underwater throughout the Hadean and much of the Archean periods. It wasn't until the Mesoarchean that continents began...

19 September 2023 6,875 0 View

Has anyone studied locus specific 5-hydroxymethylation using Epimark 5mC and 5hmC kit?

I am trying to quantify 5mC and 5hmC using Epimark 5mC and 5hmC kit from NEB. I did the end-point PCR after glucosylation and digestion with MspI and HpaII. It didnt generate a a yes or no answer,...

06 September 2023 6,221 0 View

How to calculate the number carbon quantum dots (CQDs) internalized per cell using "IntraCell V2" plugin in ImageJ?

How can the number of carbon quantum dots (CQDs) internalized per cell be calculated using the "IntraCell V2" plugin in ImageJ?

01 September 2023 419 0 View

Why the EPMA analysis for FeO for native metal (Iron) is coming as 110, 124, 114 (wt%) etc.?

The EPMA analysis for FeO for native metal (Iron) is coming as 110, 124, 114 (wt%). What could be the possible reason?

20 July 2023 2,583 0 View

What is the unit of polarization resistance (Rp) and Area Specific Resistance(ASR) for symmetric SOFC ?

I need to calculate oxygen exchange current density using polarization resistance.

07 June 2023 9,237 2 View

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How to isolate lymphocytes from mouse spleen?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

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Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

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Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

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What is the problem with these tissue culture plants?

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Found this object in the cell culture - do you have any suggestions what it might be?

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