Can you help me with pharmacopoeia procedure 3.2.1 on arsenic determination?

Hi to all I am writing you in order to get more information about the procedure 3.2.1 on Arsenic determination in glass containers for pharmaceutical use. In the monograph, at the paragraph...

05 June 2018 1,229 3 View

How do you overcome the following issues with S and Os ICP-OES?

hi to all, i'm experiencing several issues with detection of elements such as sulfur and osmium at icp-oes. in particular, i work with an Agilent ICP, and at each analysis, i have to analyse these...

08 September 2017 5,321 3 View

Is it possible to infere from isoelectric point the superficial pH?

hi to all, i would like to estimate the superficial pH on spherical particles having dimension of nanometers. since the surface is regular and omogeneusly coated, i was wandering if could i infere...

02 March 2016 8,923 2 View

No title

02 March 2016 8,187 4 View

How can I stop protein induction?

hi to all, i was wandering if it is enough to pellet cells (e.coli) from the protein induction medium containing iptg and resuspend them in a new medium without inductor to stop the protein...

01 February 2016 3,521 3 View

Is it possible to co-trasform E.Coli bl21 de3 gold with two iptg inducible plasmids?

hi to all, i was wandering if it is possible to transform bl21 de3 cells with two plasmids. the aim is to espress 2 proteins at once. the two plasmids are both iptg inducible, and ampicillin...

01 February 2016 2,988 8 View

Is it strictly necessary the chloramphenicol in LB agar plates for pLysS cells, or the ampicillin is enough?

If I add chloramphenicol only in the overnight liquid culture, is ok? should the chloramphenicol be present also during the induction of the protein? thank you

31 December 2015 1,109 3 View

Could the OD_600 of a bacterial culture be a clue of protein expression?

hi to all, i have noticed that if i incubate two parallel bacterial cultures in the same conditions ( one where i add IPTG for protein induction and another in which I don't add anything) the OD...

31 December 2015 935 7 View

Can glucose polymers be used by E.Coli as carbon Source?

Hi to all, I'm wondering if glucose polymers such cyclodextrins, glycogen and more complex polymers, are metabolized by E.Coli. Thank you

09 October 2015 2,677 4 View

How can I dock a 4Fe-4S cluster into a protein?

hi to all, I'm trying to dock a 4Fe-4S cluster into a protein using the Haddock web-tool. I suppose that the protein I'm using coordinates the cluster by means of specific residues, that I have...

05 June 2015 5,853 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View