Is there any stain which is used in place of acetocarmine?

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How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

CFD steady state simulation not converging even after large number of iterations for multiphase flow?

Dear All, I have been working on multiphase flow through a complex geometry having porosity 0.25. I have patched it with oil and high-salinity brine. At the inlet I am flowing low-salinity brine....

04 December 2020 4,506 3 View

Absolute values in Leptin ELISA kit are higher than normal. Does it depend on washing procedure?

I have been using mouse leptin ELISA kit (Catalog number (90030) from crystal chem. I have been doing manual washing between my steps as I didn't have automatic washer instrument. Does this cause...

12 August 2020 4,352 4 View

Which book/study material would be best for a beginner in optimization research field ?

power system optimization problems and optimized unit commitment

20 April 2020 6,248 12 View

How to generate a magnetic field around a sand packed column?

Hi all! I want to make a lab scale setup. I want to generate a magnetic field around a sand packed column. I want to inject magnetic nanoparticles in th sand column and want to track them...

07 April 2020 1,393 1 View

What is the best way to euthanize invertebrate insects in the field for RNA sequencing?

Field work will be conducted in very harsh, windy, and cold environment.

08 March 2020 8,387 3 View

Is it possible to remotely detecting location of fluorescent nanoparticles in a core sample using a quencher?

Dear all, I have injected fluorescent-based nanoparticle fluids in core samples. Is it possible to remotely detect the location of fluorescent nanoparticles in a core sample using a quencher? Thanks

11 January 2020 3,102 3 View

Should oil recovered after water flooding be similar for all core flooding experiments to compare oil recovered by different surfactants in CEOR?

I am performing core flooding experiments to compare percentage of additional oil recovered during surfactant flooding in a sand pack column. Is it necessary that after secondary waterflooding...

16 December 2019 2,886 2 View

Is it possible to get the two laser spots produced by using a wedge prism in a single line ?

I split the laser light into two parts by using a wedge prism, but the two spots are not in a single line. How can I make it in a single line? Is it possible to increase the separation between the...

03 December 2019 7,168 3 View

Is it possible to split a laser light (1064 nm) into two equal parts by using wedge prism? Any other optics required along with wedge prism?

I have tried to split the 1064 laser light into two equal energy spots by using a wedge prism of wedge angle 2 degree. But I cannot split the laser light. There are so many papers reported the...

12 November 2019 7,645 3 View

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Formaldehyde is used for staining the cytoplasm in the micronucleus assay.What is the reason of this?

06 July 2019 5,360 3 View

How do I calculate the %cells with micronucleus and its statistical analysis?

I am currently doing micronucleus assay in cancer cells. I am wondering on how to calculate the frequency % cells with micronucleus from the images I collected and the best approach to calculate...

11 June 2019 1,463 3 View

How do I calculate the %cells with micronucleus and its statistical analysis?

I am currently doing micronucleus assay in cancer cells. I am wondering on how to calculate the frequency % cells with micronucleus from the images I collected and the best approach to calculate...

05 June 2019 3,748 3 View

Is it possible to verify micronucleus formation in histological slides without cells dissociation?

Is it possible to stain histological slides from larger tissue sections and without dissociating the cells, with Acridine Orange or Giemsa dyes aiming to verify micronucleus formation? We intend...

04 April 2019 4,198 3 View

Is it possible to verify micronucleus formation in histological slides without cells dissociation?

Is it possible to stain histological slides from larger tissue sections and without dissociating the cells, with Acridine Orange or Giemsa dyes aiming to verify micronucleus formation? We intend...

03 April 2019 7,197 4 View

How to perform micronucleus analysis using imageJ?

I'm trying to quantify the micronucleus index in bi nucleated cells, but can't find a way to distinguish between mono, bi and multi nucleated cells. Is there any pluggin?

03 April 2019 9,908 5 View

How to perform micronucleus analysis using imageJ?

I'm trying to quantify the micronucleus index in bi nucleated cells, but can't find a way to distinguish between mono, bi and multi nucleated cells. Is there any pluggin?

31 March 2019 4,346 5 View

Why is DMSO used as a control in micronucleus assays?

I am writing up my project report and need to understand why DMSO was used as a control, when comparing Cisplatin treated HS-5 cell-lines with Cisplatin/Neutral Sphingomyelinase Inhibitor treated...

06 July 2018 3,800 0 View

What is the cause of the differences between glass microscope slides in retaining cells?

I try to optimize a micronucleus assay in cell lines (suspension cells). I switched to frosted slides from non-frosted ones, and I seems that cells do not attach properly to them. My cells are...

02 March 2018 6,653 1 View

What is the cause of the differences between glass microscope slides in retaining cells?

I try to optimize a micronucleus assay in cell lines (suspension cells). I switched to frosted slides from non-frosted ones, and I seems that cells do not attach properly to them. My cells are...

28 February 2018 7,771 1 View