Is there any stain which is used in place of acetocarmine?

More Shilpa Varma's questions See All

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

CFD steady state simulation not converging even after large number of iterations for multiphase flow?

Dear All, I have been working on multiphase flow through a complex geometry having porosity 0.25. I have patched it with oil and high-salinity brine. At the inlet I am flowing low-salinity brine....

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Absolute values in Leptin ELISA kit are higher than normal. Does it depend on washing procedure?

I have been using mouse leptin ELISA kit (Catalog number (90030) from crystal chem. I have been doing manual washing between my steps as I didn't have automatic washer instrument. Does this cause...

12 August 2020 4,352 4 View

Which book/study material would be best for a beginner in optimization research field ?

power system optimization problems and optimized unit commitment

20 April 2020 6,248 12 View

How to generate a magnetic field around a sand packed column?

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What is the best way to euthanize invertebrate insects in the field for RNA sequencing?

Field work will be conducted in very harsh, windy, and cold environment.

08 March 2020 8,387 3 View

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Should oil recovered after water flooding be similar for all core flooding experiments to compare oil recovered by different surfactants in CEOR?

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16 December 2019 2,886 2 View

Is it possible to get the two laser spots produced by using a wedge prism in a single line ?

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No title

Formaldehyde is used for staining the cytoplasm in the micronucleus assay.What is the reason of this?

06 July 2019 5,360 3 View

How do I calculate the %cells with micronucleus and its statistical analysis?

I am currently doing micronucleus assay in cancer cells. I am wondering on how to calculate the frequency % cells with micronucleus from the images I collected and the best approach to calculate...

11 June 2019 1,463 3 View

How do I calculate the %cells with micronucleus and its statistical analysis?

I am currently doing micronucleus assay in cancer cells. I am wondering on how to calculate the frequency % cells with micronucleus from the images I collected and the best approach to calculate...

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Is it possible to verify micronucleus formation in histological slides without cells dissociation?

Is it possible to stain histological slides from larger tissue sections and without dissociating the cells, with Acridine Orange or Giemsa dyes aiming to verify micronucleus formation? We intend...

04 April 2019 4,198 3 View

Is it possible to verify micronucleus formation in histological slides without cells dissociation?

Is it possible to stain histological slides from larger tissue sections and without dissociating the cells, with Acridine Orange or Giemsa dyes aiming to verify micronucleus formation? We intend...

03 April 2019 7,197 4 View

How to perform micronucleus analysis using imageJ?

I'm trying to quantify the micronucleus index in bi nucleated cells, but can't find a way to distinguish between mono, bi and multi nucleated cells. Is there any pluggin?

03 April 2019 9,908 5 View

How to perform micronucleus analysis using imageJ?

I'm trying to quantify the micronucleus index in bi nucleated cells, but can't find a way to distinguish between mono, bi and multi nucleated cells. Is there any pluggin?

31 March 2019 4,346 5 View

Why is DMSO used as a control in micronucleus assays?

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06 July 2018 3,800 0 View

What is the cause of the differences between glass microscope slides in retaining cells?

I try to optimize a micronucleus assay in cell lines (suspension cells). I switched to frosted slides from non-frosted ones, and I seems that cells do not attach properly to them. My cells are...

02 March 2018 6,653 1 View

What is the cause of the differences between glass microscope slides in retaining cells?

I try to optimize a micronucleus assay in cell lines (suspension cells). I switched to frosted slides from non-frosted ones, and I seems that cells do not attach properly to them. My cells are...

28 February 2018 7,771 1 View

Shiwani Kaushal

Carbol fuchsin , toluidine blue, feulgen are the alternatives. I would prefer you to use Carbol fuchsinas, as it is found to be more superior than the other two.

Regards

Olivier Catrice

Sulforhodamine 101, Silver Nitrate, Evan's blue ...

Arvind Singh

Feulgen is the best alternative which is a specific nuclear stain unlike Acetocarmine.

Shilpa Varma

thanks a ton....