Is there any spectroscopic method for analysis of antiretroviral drugs?

More Harshad Paithankar's questions See All

Why am I not able to separate His6-MBP from the protein of my interest?

My protein is of ~11 kDa. While eluting from the Gel filtration column, It comes with the MBP tag. I did cleavage to separate tag from the protein. So other impurities are removed but the MBP tag...

07 August 2015 978 13 View

How can I calculate the thermodynamic parameters of double stranded RNA?

I want to compare the thermodynamics of the two end of the dsRNA. Is there any software available? Is there any reference paper by which I can find the local thermodynamic parameters of the RNA at...

01 February 2015 5,571 3 View

Can any one explain how to get the dwell time plots from the FRET trajectories?

How do I convert the fret Eapp data to the dwell time?

10 November 2014 4,827 2 View

Can I use EDTA and/or DTT in wash buffer and elution buffer used for Ni affinity chromatography?

I need to purify His6 tag containing protein. Normally I use lysis buffer containing EDTA and DTT for preparing cell lysate. So can I use same buffer by adding imidazole for chromatographic...

08 September 2014 6,006 8 View

How can I purify MBP-TEV tagged protein expressed from BL21 cells?

Is there any other way than affinity chromatography to separate the protein? How much TEV to protein ratio can be used for TEV cleavage? Also can any one suggest the conditions to be used for the...

08 September 2014 7,922 7 View

Which proteins can be separated by using reverse phase HPLC?

Is there any comparative study of protein purification or separation by using reverse phase HPLC and fast protein liquid chromatography (FPLC)? What are the criteria for separation of protein by...

02 March 2014 2,263 1 View

Does anyone know any HPLC methods having a mobile phase with the combination methanol/water or acn/water?

I want to study these methods for a project. Please also give retention time and retention factor for as many drugs as available.

06 July 2013 568 3 View

is there any reference where I can get UV spectra for the various molecule?

I am interested for the UV spectra of vitamins.

02 March 2013 9,658 3 View

Can anyone tell me how to determine the LOQ and LOD for a HPLC assay method, practically?

Is there any way other than prediction from signal to noise to ratio? In case of s/n, the s/n for the peak near its base should be considered or the ratio should be calculated from the base line...

01 February 2013 5,957 17 View

How can we distinguish between different types of columns?

for example, ODS, synchronus, hypersil, nucleosil, etc.

31 December 2012 641 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Elumalai Sambandan

yes you can simultaneously estimate lopinavir and ritonavir using UV spectrophotometer as the lmax of ritonavir and lopinavir were found to be 239nm and 259nm respectively,

Teodoro S Kaufman

There are plenty of them. Now, what is your specific problem?. Lopi and rito Just google them!

Roland J.W. Meesters

Here is a paper on simultaneous measurement of LPV/r and other protease inhibitor drugs and NNRTI

http://www.ncbi.nlm.nih.gov/pubmed/12766571