Is there any spectroscopic method for analysis of antiretroviral drugs?

More Harshad Paithankar's questions See All

Why am I not able to separate His6-MBP from the protein of my interest?

My protein is of ~11 kDa. While eluting from the Gel filtration column, It comes with the MBP tag. I did cleavage to separate tag from the protein. So other impurities are removed but the MBP tag...

07 August 2015 1,027 13 View

How can I calculate the thermodynamic parameters of double stranded RNA?

I want to compare the thermodynamics of the two end of the dsRNA. Is there any software available? Is there any reference paper by which I can find the local thermodynamic parameters of the RNA at...

01 February 2015 5,631 3 View

Can any one explain how to get the dwell time plots from the FRET trajectories?

How do I convert the fret Eapp data to the dwell time?

10 November 2014 4,875 2 View

Can I use EDTA and/or DTT in wash buffer and elution buffer used for Ni affinity chromatography?

I need to purify His6 tag containing protein. Normally I use lysis buffer containing EDTA and DTT for preparing cell lysate. So can I use same buffer by adding imidazole for chromatographic...

08 September 2014 6,054 8 View

How can I purify MBP-TEV tagged protein expressed from BL21 cells?

Is there any other way than affinity chromatography to separate the protein? How much TEV to protein ratio can be used for TEV cleavage? Also can any one suggest the conditions to be used for the...

08 September 2014 7,966 7 View

Which proteins can be separated by using reverse phase HPLC?

Is there any comparative study of protein purification or separation by using reverse phase HPLC and fast protein liquid chromatography (FPLC)? What are the criteria for separation of protein by...

02 March 2014 2,317 1 View

Does anyone know any HPLC methods having a mobile phase with the combination methanol/water or acn/water?

I want to study these methods for a project. Please also give retention time and retention factor for as many drugs as available.

06 July 2013 620 3 View

is there any reference where I can get UV spectra for the various molecule?

I am interested for the UV spectra of vitamins.

02 March 2013 9,719 3 View

Can anyone tell me how to determine the LOQ and LOD for a HPLC assay method, practically?

Is there any way other than prediction from signal to noise to ratio? In case of s/n, the s/n for the peak near its base should be considered or the ratio should be calculated from the base line...

01 February 2013 6,010 17 View

How can we distinguish between different types of columns?

for example, ODS, synchronus, hypersil, nucleosil, etc.

31 December 2012 702 10 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Where to start the Analytical model for Dry sliding wear of metal surface ?

I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.

29 July 2024 6,115 0 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View

Elumalai Sambandan

yes you can simultaneously estimate lopinavir and ritonavir using UV spectrophotometer as the lmax of ritonavir and lopinavir were found to be 239nm and 259nm respectively,

Teodoro S Kaufman

There are plenty of them. Now, what is your specific problem?. Lopi and rito Just google them!

Roland J.W. Meesters

Here is a paper on simultaneous measurement of LPV/r and other protease inhibitor drugs and NNRTI

http://www.ncbi.nlm.nih.gov/pubmed/12766571