Hi buddy would you help me to know how to predict protein expression value with bioinformatic's software.
and I need to know Is there any differences between "refolding and purification" or " purification and refolding" yield.how can i optimize it ? thank you very much indeed
I am not sure about the software, but you can simply lyse the cells and bind to the resin and run SDS gel.
Can u please elaborate, what exactly u mean by 'any differences between "refolding and purification" or "refolding and purification" yield'?
Nargess, you could have a look at codon usage to find out if there might be rare codons. For a list of tools, just search the net.
You can use R (open source tool). Particularly, car or caret packages are useful.
In which organism are you expressing the gene? For prokaryotes, E. coli in particular, you can use the RBS Calculator: https://salislab.net/software/reverse
It doesn't predict absolute expression levels, but since translation initiation (TIR), which is influenced by the ribosome binding site sequence, seems to be a crucial rate limiting in protein translation, it gives a good relative hint. In my experience, genes with TIR values 100'000 can be strongly expressed. Of course this is quite relative, since gene copy-number, promoter strength, codon usage, and mRNA stability are not considered.
https://salislab.net/software/reverse
Article Automated Design of Synthetic Ribosome Binding Sites to Prec...
- Badges
- Science topic
- Similar topics
- Computer Science and Engineering
- Bioinformatics
- Bioinformatic Tools