Why do cells not attach after trypsinization?

Hi all, Currently, I am having an interesting problem. Although I worked with the cell line, dld-1, before without any issue, this time, they dont attach the flask after trypsinization. Has...

11 December 2019 3,590 3 View

Silenced gene does not affect downstream target genes?

Hi, I silenced a gene (LSD1, KDM1A) using 2 different shRNA in 3 different cell lines. When I check its mRNA level, it is more than 70-80% silenced. However, if i check its downstream target (at...

03 April 2018 5,872 3 View

Calcium Phosphate Transfection Problem?

Hi, I am trying to transfect 293T cells using 2M CaCl2 and 2X HBS (50 mM HEPES, pH 7.07, 10 mM KCl, 12 mM dextrose, 280 mM NaCl, 1.5 mM Na2PO4) However, some plates, still have particles (probably...

02 March 2018 4,689 6 View

SiRNA Transfection to COLO205, SW620, HT29?

Hi, Is there anyone who has done siRNA Transfection in COLO205, SW620, HT29 especially using either RNAiMAX or Lipofectamine3000? Besides do you have any experience to transfect/transduce plasmid...

11 December 2017 1,759 4 View

Cannot show reduced lncRNA level using siRNAs?

Hi all, I am trying to perform a loss-of-function assay. I silenced some lncRNAs which I found them from a high-throughput screening considering changes in their downstream molecule. In order to...

09 October 2017 3,855 0 View

Alternative to Southern Blot?

08 September 2016 4,045 3 View

Having problem with mutant IDH (Isocitrate dehydrogenase) enzyme overexpressed cells?

Hi, I am having trouble with cells overexpressed mutant IDH enzyme. Problem is that it does not work. In other words, I chose some genes as a positive control from other papers and evaluated their...

02 March 2016 8,254 3 View

No title

08 September 2015 4,775 2 View

How can I protect RWPE-1 cells from any contamination?

06 July 2014 8,632 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View