10 Questions 20 Answers 0 Followers
Questions related from Mert Burak Öztürk
Hi all, Currently, I am having an interesting problem. Although I worked with the cell line, dld-1, before without any issue, this time, they dont attach the flask after trypsinization. Has...
12 December 2019 3,668 3 View
Hi, I silenced a gene (LSD1, KDM1A) using 2 different shRNA in 3 different cell lines. When I check its mRNA level, it is more than 70-80% silenced. However, if i check its downstream target (at...
04 April 2018 5,938 3 View
Hi, I am trying to transfect 293T cells using 2M CaCl2 and 2X HBS (50 mM HEPES, pH 7.07, 10 mM KCl, 12 mM dextrose, 280 mM NaCl, 1.5 mM Na2PO4) However, some plates, still have particles (probably...
03 March 2018 4,753 6 View
Hi, Is there anyone who has done siRNA Transfection in COLO205, SW620, HT29 especially using either RNAiMAX or Lipofectamine3000? Besides do you have any experience to transfect/transduce plasmid...
12 December 2017 1,843 4 View
Hi all, I am trying to perform a loss-of-function assay. I silenced some lncRNAs which I found them from a high-throughput screening considering changes in their downstream molecule. In order to...
10 October 2017 3,929 0 View
Hi, i am currently looking for an experiment alternative to southern blot. I heard that pcr based assays give results quickly ( let's say within few hours). I want to perform sybr green relied pcr...
09 September 2016 4,145 3 View
Hi, I am having trouble with cells overexpressed mutant IDH enzyme. Problem is that it does not work. In other words, I chose some genes as a positive control from other papers and evaluated their...
03 March 2016 8,327 3 View
Hi, I am trying to remove Thrombin after cleavage of GST tag while protein purification. For this purpose, I have p-Aminobenzamidine–Agarose beads. I tried few conditions (such as different...
09 September 2015 4,836 2 View
RWPE-1 is a prostate epithelial cell line. I use Keratinocyte-SFM with recombinant human Epidermal Growth Factor (rhEGF) and Bovine Pituitary Extract (BPE). It doesn' t include Pen./Strep....
07 July 2014 8,695 11 View
For example, you got some DNA sequences from patient and you saw that there are some mutations. Thus, you wanted to check that sequences with possible diseases. Wıth this software/website you...
04 April 2014 8,975 4 View
