As the existing in vivo models for PCOS is time consuming and not 100% relevant. Existing in vitro models are very complex to understand thats why I am looking for a simple in vitro model that can be done easily in short span of time. Suggestions from you will be guidance to me
The model will depend on what you want to perform, one of the most relevant parameters of the diagnosis of PCOS is hyperandrogenism, which is why various in vitro models of cells have been developed in conditions of high concentration of androgens (testosterone, DHEA, etc. ). In our laboratory we have published several articles with an in vitro model of PCOS of cells stimulated with testosterone and insulin.Given the compensatory hyperinsulinemia to insulin resistance present in a high percentage of women with PCOS. I hope it helps you find the model you require!
We have been used human endometrial cell lines (T-HESC and St-T1b) for our PCOS model, since we are interest in endometria in this pathology.
No, you cant use carcinoma cell lines. You have to use normal ovarian cell line for inducing PCOS model.
Sir can you please suggest some normal ovarian cell line for inducing PCOS model
you can use ovarian granulosa cell line ( Immortalized Rat Steroidogenic Granulosa Cells Cat.No.:T0606 abm) or you can go for primary culture ( ubba et al 2016 )because immortalized GC is very costly affair.
Which drug can be used induce PCOS for invitro studies. which parameter we can study. Please suggest me.
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