Is there any simple method for the isolating and possible protection of the hypothalamic neuronal cells from mice ?
Hi: Enclosed please find two papers for hypothalamic neuron culture. One paper "Primary Hypothalamic Neuron Culture--Dissociated via Papain" show the protocol for isolation of hypothalamic neuron. This is a convenient and simple protocol for hypothalamic neuron culture. Another paper describes the mass immortalization
of mouse primary hypothalamic cells in mono-layer culture to generate a vast representation of hypothalamic cell types have been further characterized by analysis of 28 neuroendocrine markers. These cell models can be used for detailed molecular analysis of neuropeptide gene regulation and signal transduction events involved in the direct hormonal control of unique hypothalamic neurons.
Hi: Enclosed please find two papers for hypothalamic neuron culture. One paper "Primary Hypothalamic Neuron Culture--Dissociated via Papain" show the protocol for isolation of hypothalamic neuron. This is a convenient and simple protocol for hypothalamic neuron culture. Another paper describes the mass immortalization
of mouse primary hypothalamic cells in mono-layer culture to generate a vast representation of hypothalamic cell types have been further characterized by analysis of 28 neuroendocrine markers. These cell models can be used for detailed molecular analysis of neuropeptide gene regulation and signal transduction events involved in the direct hormonal control of unique hypothalamic neurons.
thanks and regards Chao Hsu-Wen
I will check hope that it would be good for me
thanks again
We do this routinely with primary rat hypothalamic neurons. The cells are very sensitive so we have found that it is quite important to follow the protocol closely. You can find the details of the methods here:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774346/
Hello Jessica,
Do you have images of the dissection part?
Cheers, Laura
Hi Laura,
No, we do not have images of the dissected area, although we describe how we took it in the paper. We use the Altman and Bayer 1995 prenatal rat atlas to determine our area. Here is the description from the Methods:
"The brain was sliced with the ventral side up using a no. 11 scalpel. Two coronal cuts were made anteriorly at the level of the optic chiasm and posteriorly at the level of the mammillary bodies. This slice was then oriented coronally, and the hypothalamus was dissected as a rectangle, with the dorsal cut made ~2 mm from the dorsal aspect of the slice and the lateral cuts made ~1 mm from the medial aspect of the slice."
If you can get a hold of the atlas, that should be pretty clear. Good luck.