How to run Wavelet analysis in Python or R or Matlab?

Can anyone guide by providing the procedure to run and interpret Wavelet analysis in Python or R or Matlab? Thanks in advance

09 May 2020 3,778 8 View

Help me with this doubt - PCR mixture??

Two PCR mixtures, 55 and 60 micro litre total volume. The mixture contains 10 and 15 micro litre of DNA template respectively. PCR for Panfungal genome targeting ITS was performed. Reaction was...

02 July 2019 9,520 3 View

Anyone can please tell me how to simulate natural and hybrid woven fibre laminated composite subjected to low velocity impact by using ANSYS LS-DYNA?

20 August 2016 9,802 1 View

In gelpolymerelectrolyte peoples always use polarpolymer like PVDF,PMMA,PAN,PLAetc for separator.why not reamaining polymers likePE,PP,PET,PC,PVC,etc?

i need to know why researcher always used this polar polymer for gel polymer electrolyte for Li-ion battery.still i cant get answer.

24 February 2016 5,859 2 View

How can I dissolve PLA+chitosan for miscible blends? How many grams can I use to form a proper solution concentration?

i want to blending of PLA with chitosan for miscible blending.i need concentration value or hoe much grams of polymer i need to dissolve for the suitable solvents.

12 October 2015 7,795 3 View

How to stain the Glial cells of CNS by routine histological procedures?

How to stain the Glial cells of CNS by routine histological procedures? Thanks in advance

20 January 2015 7,497 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View