Is there any researcher working on fluorescent dyes Rhodamine 6G, FITC Dextran 500,000 MW?
I would like to know the IV dose in Rat.
I want to use it in vivo for WBC detection.
Thank you
Hello Team, I would like to calculate the strain at a node #5 in a direction (l1,m1,n1) in the turbine blade FE model as shown. This node is connected with four elelemnts (#1,#2,#3,#4) and I have...
11 January 2021 7,702 3 View
One invention granted for the US and European patent will be counted as one patent or 2 patents for CV writing?
05 January 2021 8,135 4 View
Can anyone help me with the FEM coding for Buckling of composite plates using the FSDT or HSDT - Source or the helping material (textbook, research paper etc) - Geometric stiffness matrix...
03 January 2021 3,059 3 View
In Gausssian, I have tried to reoptimize that structure by freezing the bond which is breaking in excited state during optimization. Before freezing the bond i have already tried scf = xqc and scf...
20 December 2020 8,444 3 View
I am doing my Ph.D. in Materials Science and am completely new to Molecular Dynamics Simulation and Linux. I am using Manjaro Linux 20.1 and have Gromacs 2020.4, VMD 1.9.3, packmol 20.0.10,...
28 October 2020 1,051 3 View
Hi This is Ravi. i am trying to create a separation bubble on a flat plate using a contoured upper wall. I want to apply same pressure distribution of a given blade on the flat plate. I have read...
21 October 2020 6,635 5 View
Can someone suggest to me a good and not so expensive Fluorescence camera for intravital microscopy of blood vessels? or can someone advise me on choosing the right camera for intravital...
15 October 2020 4,461 1 View
The moment-curvature plot in SAP2000 is based on the material stress-strain plot of caltran (along with its material FoS). I would like to understand the detailed calculation to obtain the plot...
13 August 2020 5,729 3 View
Any one who are in that track kindly give an idea of how to forward in this idea. Thanking you all
26 July 2020 2,898 3 View
I am working on III Nitride multi-quantum well light-emitting diodes using Crosslight APSYS. Here I would like to extract the electron and hole wave functions, Can anyone please suggest how to...
19 June 2020 8,097 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View
Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...
22 February 2021 592 1 View
I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...
15 February 2021 2,830 2 View
Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...
15 February 2021 4,353 3 View