Like in the headline. We have found a publication with PAP (prostatic acid phosphatase) but it seems that it doesn't work - too many differences between individuals.
I want to compare the expression of some proteins in human seminal plasma with Western blot.
Dear Karolina,
as far as I know there is not a reference protein for plasma analysis by western blot. In our work and in literature, what I see is that you compare plasma by "loading control" with Ponceau staining.
Hope I could help.
Hello!
I'm having the same question... Karolina, what did you do in the end? Did you find any internal control or anything to compare different seminal plasma samples by WB?
Thanks a lot
We compare it to total protein. After electrotransfer you can die your membrane with i.e. ponceau, take a picture of it and calculate the amount of total protein in a single row. Personally, we use stain free gels (form BioRad) and using ChrmiDoc system we visualize proteins in the membrane. The software compare the picture with total protein and, after immunobloting, with your protein.
:)))
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