Is there any reason people use T7E1 assay more often compared to the disruption of restriction enzyme recognition site when analyze NHEJ by CRISPR?

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How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Does the property of GFP affect a fusion protein's localization?

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15 February 2021 4,353 3 View

Can part of recombinant protein not be expressed?

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14 February 2021 2,039 4 View

How can I prevent bubbles from forming under a coverslip?

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10 February 2021 3,580 1 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

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09 February 2021 9,012 3 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

Why E1g mode (Molybdenum disulfide) is undetectable in Raman spectroscopy?

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04 February 2021 6,580 2 View

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How do they fill/replace metal hydrides in vessels?

Surely they will want to avoid contact with air/moisture, so what is the procedure for this?

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Is it possible to mix TCEP-HCl and NaI chaotropic reagent?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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03 March 2021 3,568 1 View

How to quantitative analize the data of TNBS assay for gelatin?

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to simultaneously perform multiple biochemical assays?

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Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

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What could be some reasons why unstimulated monocytes are getting activated?

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RIA. How to measure?

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Amanda Edson

I have used this method very successfully. Especially as it gives you a unique finger print for WT (one band-cut), hetero mutant (2 bands), and homo mutant (one band- uncut). I haven't seen a downside.

Mahdi Moradpour

Generated mutations through NHEJ can be detected either by PCR/RE assay or T7E1 assay.

The T7E1 assay would be used when there is no appropriate RE sites.

Kok Zhi Lee

Thanks Amanda for your answer and Mahdi for additional input.