Kok Zhi Lee

2 Questions 7 Answers 0 Followers

Questions related from Kok Zhi Lee

Does anyone familiar the restriction modification system in DH10B and DH5alpha E.coli strain?

I have plasmid DNA (BFP in pET vector) that minipreped from DH10B and DH5apha. There is no colony when I transformed DNA (from the DH10B) to MG1655, and many colonies when I transformed DNA (from...

18 September 2019 6,331 8 View

Is there any reason people use T7E1 assay more often compared to the disruption of restriction enzyme recognition site when analyze NHEJ by CRISPR?

Besides having a unique restriction enzyme site, is there any downside of using disruption of restriction enzyme recognition site as a method to check if there is NHEJ?

09 April 2018 3,005 3 View