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I have plasmid DNA (BFP in pET vector) that minipreped from DH10B and DH5apha. There is no colony when I transformed DNA (from the DH10B) to MG1655, and many colonies when I transformed DNA (from...
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Besides having a unique restriction enzyme site, is there any downside of using disruption of restriction enzyme recognition site as a method to check if there is NHEJ?
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