Dear Colleagues and Friends
I have faced a strange results in my previous experiment.
I had Melanoma cells in culture and after 4 days, I wondered when I saw that the melanoma cells were morphologically changed to a neuron like cells or more likely to melanoblast cells.
The most exciting part of this morphological changes, that prevent me to ignore, was that their growth have been stopped or significantly decreased without applying any specific/new chemicals.
So, I would like to know about any non-specific reason behind this phenomena.
Considering that, this morphological and metabolic changes were happened for a malignant/invasive type of skin cancer cells, the answer may results in a way to step forward in cancer treatment.
Best Regards
Behnaz Ghaemi
This sounds to me that they were sub-cultured too sparse and did not recover well. What cell to flask ratio did you use?
Dear Heather
I have checked the cells after 24h post sub culture, they had the normal morphology, but during 4 days they changed to a diffrent morphology.
A noticeable change in cell morphology that associated with a cell growth arrest often results due to sub-optimal cell culture conditions. These conditions include, a change in cell density, culture medium (growth factors), cell culture flask/plate (a change in extracellular matrix-induced signaling), and sub-optimal trypsinization of cells. Other conditions (the list is long) are also known to alter the cell morphology. Therefore, if you have notes from your cell culture conditions, it should be possible to identify potential factor(s) that might have contributed to your observations.
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