I want to study effect of phytochemical on adipocyte differentiation currently we have NIH/3T3 not 3T3-L1 so is there any method I can differentiate 3T3 to adipocyte. I have no time to order a 3T3-L1 now.
No. NIH-3T3s will never work for adipogenic differentiation unless you force expression of an adipogenic priming transcription factor (e.g. TCF7L1, PPARG).
That is why 3T3-L1s were such an important discovery when initially isolated- because they have differentiation potential that is missing in many fibroblastic cell lines including NIH-3T3s.
Totally agree with the previous answer. Don't waist your time on NIH 3T3 cells. Buy 3T3L1 cells from the ATCC.
The following paper would be useful and is in line with the previous replies. NIH-3T3 do not differentiate into adipocytes
Anal Biochem. 2012 Jun 1;425(1):88-90. doi: 10.1016/j.ab.2012.03.005. Epub 2012 Mar 13.
Protocol for effective differentiation of 3T3-L1 cells to adipocytes.
Zebisch K1, Voigt V, Wabitsch M, Brandsch M.
NO. There is a paper to show differentiating 3t3/NIH to adipocyte. I followed this protocol and it worked properly.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0051459
Please see this paper.
Without any information to authenticate the cell line as NIH-3T3s, it is hard to interpret this paper as bona fide evidence that NIH-3T3s can differentiate to adipocytes. It is interesting to learn that you observed similar results using the published protocol, Yong.
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