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11 January 2021 3,284 3 View

How can we count the number of edges in an edge detected image using canny/sobel algorithm in MATLAB?

I need to process an image for edge detection and then want to count the number of edges in that image.

29 December 2020 5,594 2 View

Which are the vendors who sell commercial SWIR (NIR-II) dyes?

I am searching to find commercial vendors who sell SWIR dyes. I could only find Nirmidas Biotech which sells different NIR-II dyes!

23 December 2020 5,002 3 View

How to get doi of presentation ?

doi of your presentation in the conference

23 December 2020 9,370 2 View

Precipitation of Pcl from polymeric solution ?

We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?

09 December 2020 5,033 11 View

How to induce doxorubicin resistance in MCF-7 and MDA-MB-231?

I want to establish DOX/R cell line in vitro for the research study.If any one suggest the protocol then it would be a great help.

03 December 2020 9,795 5 View

What is the difference between weak from Ritz method and variational form Ritz method while solving a boundary value problem ?

What is the difference between weak from Ritz method and variational form Ritz method while solving a boundary value problem ? Can all differential equations take variational form ?

18 November 2020 2,341 2 View

What could be the reason for a broad peak of ADC(Antibody Drug Conjugates) using HIC-UV method (Hydrophobic Interaction Chromatography)?

Hi, We are working on DAR (Drug to Antibody Ratio) estimation of ADC using the following HIC-UV method. For some of the unconjugated mab we getting a sharp peak but for our desired mab we are...

15 November 2020 7,359 0 View

Can SEM-EDS give a chemical formula?

I have attached an excel file with the quantification of a mineral I conducted EDS on. I have more excel files of minerals too as I know EDS spectra for only 1 sample will certainly not be...

03 November 2020 6,899 9 View

Can TBDMS protecting group be cleaved under 25% TFA/DCM?

I performed a boc deprotection via 25% TFA/DCM (1 hr) without any workup, which should yield TFA salt as a product. My compound also had a TBDMS group on it. But when I took an NMR, the amine peak...

21 October 2020 4,470 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View