I am performing biomarkers analysis in samples of mussels' digestive glands. I homogenize the tissue in phosphate buffer [100 mM (K2HPO4/KH2PO4), 150 mM KCl, 1 mM DTT, 0.1 mM PMSF, 1 mM ethylenediaminetetraacetic acid (EDTA); pH 7.4]. Following protein determination (Lowry method) we perform aliquots (100 µl) of the homogenate and use them to determine GST, SOD and CAT activity as well as lipid peroxidation.

We aim to evaluate metallothioneins (MT) but we don´t have any standardize protocol at our lab. I found some papers for MT analyses by spectrophotometry, but the homogenizing protocol is different from the one that we use. Does anyone know if we can use our material (aliquots obtained from the above described method and used for the other biomarkers) for MT analysis? D you have knowledge of a protocol for microplate reader analysis?

Thank you very much.