Hello,
According to FET protocol (OECD TG 236, 2013) 20 embryos (1 per well) is an adequate number for LC50 calculation. Embryos are treated as independent replicates. The same as been corroborate by a validation study to the OECD protocol. This protocol is not directly proposed to analyse zebrafish embryo developmental anomalies, which is the objective of my work. In these context, I have the doubt if 20 embryos are a sufficient n, if they are really independent replicates and what kind of statistical analysis should I performed. Can anyone specialized in FET help me with these doubts. Thank you very much.
Joana
I think 20 embryos will serve our purpose of zebrafish embryo developmental anomalies. But as a rule the higher the sample size will strengthen your study in terms of generalization.
Hello Hamed,
Thank you very much for your anwers and recommendation.
Best regards,
Joana
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