My protein present in 8M Urea, 1mM DTT, 1mM EDTA, 1mM PMSF, 10mM Nacl & 50mM Tris @pH 8.0

And it is buffer exchanged with 50mM Tris, 10mM Nacl, 1mM DTT,EDTA & PMSF @pH 8.0

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