Is there any other way for the preparation of T4 bacteriophage ghost other than osmotic shock technique ?

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How is DNA labelled with radioactive sulphur? What are the advantages of this method over radiolabelling with phosphorous?

Labelling of DNA with radioactive sulphur

07 August 2014 2,504 3 View

Multiple ligands in autodock?

Can I dock multiple ligands in Autodock (either sequentially or simultaneously)?

07 August 2014 7,969 7 View

phage Infection

What are the Biochemical changes observed during Phage infecting bacteria ? One of them is membrane potential is anything else in the list ?? Thanks in Advance, Ganesh Shastry H M

06 July 2014 1,659 0 View

Can anyone tell me about the force involved in DNA ejection in bacteriophages?

I tried to understand both The Continuum Mechanics Model and Hydrodynamic model for DNA ejection but I cannot able to draw major differences other than the osmosis involved in hydrodynamic model....

06 July 2014 3,500 2 View

Why T4 bacteriophage tail protein in SDS resistant ?

T4 bacteriophage.

04 May 2014 9,854 0 View

How can I clone and express 3Kbp of DNA?

How can I clone 3Kbp of insert into a vector and to express the protein in E coli system?

03 April 2014 7,523 2 View

Is there any assay to detect the T4 bacteriophage ghost in the given sample other than Electron microscopy?

Detection of T4 bacteriophage ghosts

02 March 2014 1,014 0 View

What is the best minimal media for E.coli strain B 11303(ATCC)?

I have to grow E.coli in a minimal media but I don't have M9 salt to prepare minimal media. Is there any other way to make minimal media for this particular strain? Thanks in advance.

02 March 2014 8,738 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

Does broad spectrum phage become narrow spectrum by time ?

In the host range analysis, I saw that a temperate phage that I isolated had an effect against a different strain, but later (2 years) it did not affect that strain. What could be the explanation...

25 June 2024 9,729 3 View

Why is my phage library obtained at round 2 of Biopanning viscous?

After incubation of the TG1 cells with the helper phage, I spun down the culture and precipitated the supernatant with PEG/NaCl. After this, I spun it down again and resupended the pellet in PBS....

21 June 2024 6,260 2 View

Has anyone used or is familiar with an anti-C4 complement factor antibody for Western Blotting?

Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...

17 June 2024 6,124 1 View

How to Construct Synthetic Phage Library?

Hi, I am trying to make synthetic phage library. Right now , do people still use Kunkel method or other methods? Since it looks like Kunkel method is an old method. I am looking for more...

09 June 2024 6,780 1 View