I have to grow E.coli in a minimal media but I don't have M9 salt to prepare minimal media. Is there any other way to make minimal media for this particular strain?
Thanks in advance.
Dear Ganesh,
if you have the salts which are included in M9 salt mix, you can prepare minimal media from them. Minimal Medium for E. coli (grams/ liter)
Glucose 5 g
Na2HPO4 6 g
KH2PO4 3 g
NH4Cl 1 g
NaCl 0.5 g
MgSO4 0.12 g
CaCl2 0.01 g
Thank you :-) Jouni, It was very helpful is there a possibility to culture the E.coli cells in salt free media ?
If you mean a true minimal medium then you need to provide the inorganic elements that are needed for growth, such as ammonia, sodium, phosphate, carbon, chlorine, magnesium etc (plus of course an organic energy source). Those are the critical components in the minimal medium. It is certainly possible to use a solution buffered with Tris or Mops instead of phosphate buffer such as M9, but you still need to add the other salts to provide the essentials. If you use a complex medium with tryptone or peptone or yeast extract, then those inorganics are present in the macromolecules from the complex media.
Since you have not specified, what is the purpose of the minimal medium you are searching, it will not be possible to give you any specific composition. Let me tell you that you do not always need M9 salt to prepare such a medium. You may be surprised that even 1% peptone and 0.05% NaCl too is a minimal medium for E.coli B11303 (from my experience).
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