How can I clone and express 3Kbp of DNA?

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How is DNA labelled with radioactive sulphur? What are the advantages of this method over radiolabelling with phosphorous?

Labelling of DNA with radioactive sulphur

07 August 2014 2,504 3 View

Multiple ligands in autodock?

Can I dock multiple ligands in Autodock (either sequentially or simultaneously)?

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phage Infection

What are the Biochemical changes observed during Phage infecting bacteria ? One of them is membrane potential is anything else in the list ?? Thanks in Advance, Ganesh Shastry H M

06 July 2014 1,659 0 View

Can anyone tell me about the force involved in DNA ejection in bacteriophages?

I tried to understand both The Continuum Mechanics Model and Hydrodynamic model for DNA ejection but I cannot able to draw major differences other than the osmosis involved in hydrodynamic model....

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Why T4 bacteriophage tail protein in SDS resistant ?

T4 bacteriophage.

04 May 2014 9,854 0 View

What is the best minimal media for E.coli strain B 11303(ATCC)?

I have to grow E.coli in a minimal media but I don't have M9 salt to prepare minimal media. Is there any other way to make minimal media for this particular strain? Thanks in advance.

02 March 2014 8,738 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Where should the ARS sequence be introduced on a plasmid?

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05 August 2024 1,573 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

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Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

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Toshisangba Longkumer

What actually is your problem? Is it that you can't insert in a particular vector or you don't know which vector to use?

Ganesh Shastry

I have to express a viral gene which is of 3Kbp in E.coli I don't know which plasmid to use because the I have never worked with such a big insert