How can I clone 3Kbp of insert into a vector and to express the protein in E coli system?
What actually is your problem? Is it that you can't insert in a particular vector or you don't know which vector to use?
I have to express a viral gene which is of 3Kbp in E.coli I don't know which plasmid to use because the I have never worked with such a big insert
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T4 bacteriophage.
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I have to grow E.coli in a minimal media but I don't have M9 salt to prepare minimal media. Is there any other way to make minimal media for this particular strain? Thanks in advance.
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When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
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Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
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