We are working on preparing a student lab. manual with the supplies in hand. To determine how much of the glucose is metabolized by bacteria in a broth, DNS method is recommended. The problem is we do not have 3,5 dinitrosalicylic acid in our lab, which has forced me to search alternative methods. Can you recommend any other spectrophotometric method we can use instead of DNS?
Thank you in advance!
I'm not an expert in the field but I am currently doing the same review for reducing sugars analysis in plant material.
Besides DNS assay, I've found two protocolos for measuring reducing sugars:
Here you can find the original references, but I'm sure you can find a description of the protocolos elsewhere.
- Robyt, J. F., and W. J. Whelan. (1968). The a-amylases. In.: J. A. Radley, ed., Starch and its derivatives. 4th edition. Pp. 430-470. Chapman & Hall, London, England.
- Waffenschmidt, S., & Jaenicke, L. (1987). Assay of reducing sugars in the nanomole range with 2, 2′-bicinchoninate. Analytical Biochemistry, 165(2), 337-340.
Article Assay of Reducing Sugars in the Nanomole Range with 2,2′-Bic...
There are many methods for estimating reducing sugars..You can use following method-
Determination of reducing sugars by Nelson-Somogoyi method....Nelson (1944)
This is quite reliable method.
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