I am trying to develop xenograft but having no luck and the age of mice is increasing everyday
Hello Neha Masarkar
It depends upon the type of cancer cell specific features used in the xenograft model. To explain this in a better way, I have attached an article for your reference.
Article The effects of aging on tumor growth and angiogenesis are tu...
In this article, B16/F10 melanoma, a cancer cell line showed that B16/F10 tumors grew minimally in the aged mice. In contrast to B16/ F10, TRAMP-C2 tumors had an extracellular matrix with significantly higher levels of MMP2 and MMP9 expression and activity. These results demonstrate that tumor progression can be as robust in aged tissues as young tissues.
B16/F10 tumors had minimal evidence of an extracellular matrix and were comprised primarily of sheets of tumor cells. In conjunction with the presence of a well formed matrix, the TRAMP-C2 tumors had significantly greater amounts of gelatinase (MMP2 and MMP9) expression and activity.
The high expression of MMP2 and MMP9 in TRAMP-C2 tumors provides a potential explanation for the observed differences between the growth of B16/F10 and TRAMPC2 tumors in aged mice.
According to the investigators findings, they have demonstrated that tumor-cell specific features determine the effect of aging on tumor growth and angiogenesis.
Best.
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