Is there any ophiuroid specific universal primer for barcoding mitochondrial COI gene sequence?

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In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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How to calculate the annealing temperature of a primer pair?

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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Genome-wide gene identification protocol ?

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MiRNA qpcr problem?

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Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

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Sabine Stöhr

Have you read Hoareau and Boissin (2010)?https://www.researchgate.net/publication/51119903_Design_of_phylum-specific_hybrid_primers_for_DNA_barcoding_Addressing_the_need_for_efficient_COI_amplification_in_the_Echinodermata

Article Design of phylum-specific hybrid primers for DNA barcoding: ...

Chamundeeswari Kanagaraj

Thank you Dr. Sabine. I have gone through this paper and this guided me lot in finding designing primers but this paper was published during 2010. I want to know any publication regarding this after 2010 or any modified protocols after 2010?.