Is there any one peeled-off perovskite surface for STM study??

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What descriptive statistics are relevant for my project?

I am doing a project - comaprison of ethnic minority and majority mental health in terms of their past experiences of racism, discrimination and bullying. Im unsure what descriptives I need and...

04 March 2024 3,320 1 View

Can we perform RFLP on PCR product extracted from agarose gel electrophoresis?

I have performed RFLP for WT and mutant sample. The WT sample was only PCR product, while mutant band was extracted from agarose gel electrophoresis (it was checked on gel again to see the...

17 January 2024 6,427 4 View

Is there legal research listing legal cases of minority or immigrant children placed with other cultural foster homes?

I am trying to research legal cases where non-indigenous and non-caucasian children are apprehended from their families and whether culture plays any significant factor in their temporary placement.

25 November 2023 215 0 View

What can authors do if they are publishing in open access journals but their instution is not covered in the APC waiver fee list?

I am preparing a research article on public health and health promotion for a BMC Public Health journal which is open access. My university is not a member of the Springer Nature membership for...

07 September 2023 6,474 2 View

I am looking for an accurate method for determining the IC50 value?

I have used different methods including graphpad prism, excel and various other online tools but i get different values each time. I want to know how IC50 can be determined from % inhibition and...

02 October 2022 1,111 4 View

How can I elute protein from immunoprecipitated beads to use for mass spec?

Hi, I have purified my protein of interest using the specific antibody and pulled down the protein-Ab complex with protein A beads. I need to perform mass spec on my protein and need a protocol...

02 December 2021 2,015 1 View

What is the impact of issuing excessive IPOs in capital market?

In recent time in Bangladesh so many IPOs are issued.

21 October 2021 3,331 7 View

Reflexive Thematic Analysis by Braun & Clark?

Hi everyone! I have planned to use Reflexive Thematic Analysis by Braun & Clark for data analysis in my qualitative research. This method involves reflexive practice and is different from Thematic...

29 June 2021 5,668 7 View

I have forgotten to centrifuge the primer tubes before resuspension in water. Will these primers work or I need to order new?

Primer dilution issue

31 May 2021 901 3 View

Does using a likert scale in my qualitative research will make it mixed method?

I am conducting a qualitative research and using two qualitative data collection tools (1. Reflective journal 2. Semi-structured interviews). In the reflective journal I have asked the...

10 December 2020 4,921 7 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Please guide me how to prepare samples for zeiss confocal microscope?

i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...

30 July 2024 2,349 1 View

I have synthesized a composite of ZnO and In2O3 with variations in ZnO content. In Raman , what is the peak observed at 130 cm-1?

Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...

28 July 2024 5,410 1 View

Bootstrapping in SEM Amos ?

Can a researcher use small sample size of 40 while using SEM with bootstrapping of 1000 and is it possible to get published

28 July 2024 4,402 2 View