Does using a likert scale in my qualitative research will make it mixed method?

I am conducting a qualitative research and using two qualitative data collection tools (1. Reflective journal 2. Semi-structured interviews). In the reflective journal I have asked the...

10 December 2020 4,672 7 View

Is it possible to use EEGLAB (matlab toolkit) to extract features?

In my Final year project, I'm working on EEG emotion detection. I want to extract features from DEAP DATASET to detect emotions(happy, sad, depress etc.). I'm using EEGLAB for preprocessing, it...

23 October 2020 569 3 View

How Carpooling will reduce Traffic Congestion? I am researching on this in Islamabad.What will be methodology to find out facts & figure?any software?

How Carpooling will reduce Traffic Congestion? I am researching on this in Islamabad. What will be methodology to find out facts & figure? Can any one suggest best software or Survey link...

13 October 2020 8,777 8 View

General predctive control implementation in matlab ?

What is the easiest method to implement the GPC in Matlab? After the development of the GPC algorithm I got the equation on the image being U(t) the control signal and y(t) the output signal and...

22 September 2020 4,040 1 View

How do i use year, industry and country fixed effect in a single regression ?

I am doing an analysis on panel data of 30 countries. I want to control year effect, industry effect and country effect in a single regression. Please explain how can I used these three in a...

18 July 2020 7,615 5 View

Different types of clouds have different impacts on the Earth's climate?While some types of clouds help to warm the Earth, others help to cool it,How?

Currently, the combined effect of all clouds is one of net cooling, meaning that clouds are dampening the rate of climate warming. But scientists are looking into whether clouds will have the same...

09 May 2020 9,367 19 View

Can Carpooling Project could be a Final Year Project or Research Topic in Civil Engineering?

Can Carpooling Project could be a Final Year Project or Research Topic in Civil Engineering?

29 March 2020 3,825 7 View

How a deep learning approach detect that the pneumonia is caused by Covid-19 from an x-ray?

24 March 2020 6,877 3 View

The Pectin / Polysaccharide Ratio impact on texture in seed-derived food matrix ?

For a project where we're trying to understand how some crop varieties aren't so adapted to the transformation process for Mustard paste (Sinapis alba & brassica juncea). Between two different...

05 November 2019 6,867 1 View

In TEM sample preparation what quantity of nanoparticle needs to be mixed in ethanol before ultra-sonication? min quantity that gives results?

I have to send sample in another city for TEM analysis, they said send prepared sample..

25 June 2019 5,113 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

During an anti-His capture ELISA has anyone had issues with histidine residues from secondary antibodies non-specifically binding?

Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...

07 February 2021 7,101 1 View

How do I keep my prefixed (PFA) frozen tissue sections from lifting off the slide?

I'm trying to stain mouse lung tissue IHC-IF I fix in 4% PFA overnight immediately upon harvesting tissues Then dehydrate/cryoprotect in sucrose gradients 15% to 30% Then freeze in OCT media...

06 February 2021 2,336 3 View