Is there any method for the isolation of Taphrina maculans causing turmeric Leaf blotch?

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J. C. Tarafdar

@ Harshil, please have a look of the attached files.

Paul Reed Hepperly

The Taphrina maculans as pathogen of turmeric has issues with bacterial contamination.

The media recommended from Butler the original author is acidified clarified potato dextrose agar or turmeric leaf decoction agar.

The isolation technique to get a pure culture is to invert the plates and fix the necrotic lesions which are fixed on the top with a fixative and the injection of the spores can give cultures freer of bacteria.

The cultures are incubated at 20 C and alternating light is used to help stimulate the sporulation.

The fungi has yeasty conidia and ascosporic stages.