I am looking for an optimized assay, preferably bioassay kit to access iron transport in cell lines through transferrin receptor 1. If anyone knows about this kind of assay, please let me know.
The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. Target-specific ELISA kits are available from a variety of manufacturers and can help streamline your immunodetection experiments.
Ready-to-use ELISA kits are available for the detection of TFR1, an alias of the protein, transferrin receptor. The official gene symbol is TFRC. The protein, with a mass of 84.9 kDa and length of 760 residues, is reported to be membrane-associated and secreted. It is a member of the Peptidase M28 family, M28B subfamily and may be known by other names, including: TR, TFR1, T9, IMD46, CD71, and transferrin receptor protein 1.