Is there any information about RNA modifications that provide stability against RNAse ? Or any RNA subtype that is resistant to RNAse.

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Oleg Laptenko

If you plan to synthesize you RNA, then yes, there are multiple ways to increase the resistance of your RNA against a given RNase. The simplest ones are based on the inclusion of modified NTPs. Look at TriLink biotech or Oligos etc websites for example. With endogenous products it is way more difficult, mostly because of limited yield of modified RNA produced in the cell.

Ganesh Shelke

Thanks Oleg for the information. But i am looking for endogenous RNA system of cells that have such degree of protection.

Alexander Rosenberg

Ribosomal RNA and tRNAs are very stable. I've linked a paper that discusses RNA stability in bacteria.

Double stranded structures on the ends of the RNA molecule seems to increase stability as well (presumably by blocking ssRNA exonucleases). See the second linked paper

http://nar.oxfordjournals.org/content/34/2/659.long

http://nar.oxfordjournals.org/content/34/20/e138.short

Thanks alot Alexander.

Indeed prokaryotic system is evolved with such a degree of protection against RNase (that have both endo and exonuclease activity). But such degree of protection can be removed by Proteinase K treatment followed by RNAse. I am having hard time finding such system (in Eukaryotic system) that can provide stability against such condition (PK + Rnase). This could be due to many reasons....one could be vesicles, PK resistant Protein-RNA complex.. Just need the confirmation that such system exist...