No, that's probably the reason researchers used various methods in one assay to do so, like DPPH, NO, TEAC, FRAC, and TAC.
There is no single ideal method to measure antioxidants in herbal plants because different assays (e.g., DPPH, FRAP, TEAC) detect different antioxidant mechanisms. Using multiple complementary methods provides a more comprehensive and reliable assessment.
No specific assay to carry out antioxidant using herbal plants we can use same DPPH ,FRAP assays but u have to have std as ascorbic acid and one blank taking methanol
An ideal antioxidant assay does not exist. Before use an assay, you have to learn what is the chemistry behind the assay. The assay should be based on physiological related radicals under physiological conditions. For water soluble antioxidants the conditions are i) neutral pH; ii) room or about 40C; ii) ROS:
OH. HO2. O2-. ONOO-. For lipophilic i) Light paraffins as a solvent and ROOH, RO2. radicals.
Rama Shankar
Any antioxidant assay is based on certain chemical reaction(s) but not on "using herbal plant."
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