I would like to address whether my genetically engineered human AD293 cells are more or less attacked by T-cells. Is there a human T-cell line that can be used for a cytolysis assay?
Why not use T cell isolated from volunteers. Just like mixed lymphocyte reaction. The cells line are convenient but may not show the truth. Good luck.
I've been trying to find an explanation for why bandgaps can form in periodic elastic laminates but so far I haven't found a good explanation. Can anyone point me to any resources which might...
01 May 2024 2,241 1 View
I am aware that periodically layered media (if structured properly) can produce bandgaps, however I have never found a great explanation for this in the phononics case. Can someone please provide...
30 April 2024 9,681 4 View
I observed that activated carbon is employed as anode materials and manganese oxide as a cathode material for supercapacitor application using two-electrode system in KOH electrolyte. Please...
26 April 2024 7,962 1 View
It is observed that MnO2 stores charge by means of potassium (+) ion while cobalt oxide store energy by hydroxide (-) ion while both materials are employed as cathodes in separate systems.
26 April 2024 8,079 1 View
I am doing a literature review on acoustic properties of layered media and I am struggling to find articles, resources etc to add to the review. Does anyone know of any seminal papers in this...
22 April 2024 9,870 4 View
importance
18 March 2024 6,521 2 View
Those wanting full text of "Poisson's equation solution of Coulomb integrals in atoms and molecules†" May 2006 Molecular Physics 104(9):1385-1389 DOI: 10.1080/00268970500462248 📷Charles...
10 March 2024 1,273 0 View
I need the full Book for these models by Nygaard, Theoretical Model of Research Productivity And Technology Acceptance Model by Davis, 1989
05 December 2023 7,201 0 View
I need this to justify a survey sample Size.
13 November 2023 4,083 3 View
their research gaps, factors they reserched and findings
20 October 2023 2,486 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View