Is there any fully automated microfluidic chip?

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Is there any way to calibrate transducer without needle hydrophone?

Is there any way to calibrate transducer without needle hydrophone? Also any one know low cost hydrophone as precision acoustic is quite costly.

16 December 2020 1,070 2 View

How to convert mV output into MPa?

I am working on acoustic characterization of microbubbles using two transducers arranged at 90 degrees. During calibration, I am receiving response in dBm or mV. How I can know the PNP of my...

14 December 2020 1,814 5 View

What is the dielectric constant(Real and Imaginary parts ) of MASnI3 for 300 to 1200nm wavelength?

What is the dielectric constant(Real and Imaginary parts ) of MASnI3 for 300 to 1200nm wavelength? as I need it for Perovskite solar model design in Comsol Multiphysics

25 November 2020 1,350 1 View

Is there any way to prepare Polymeric Nanoparticles without adding water??

I am making Poly Glycerol Sebacate nanoparticles by solvent evaporation and nanoprecipitation, but material got hydrolyzed upon adding water. I want to replace aqueous phase. Which chemical should...

21 October 2020 8,319 9 View

Path to node for column diameter while linking Aspen with excel or Matlab?

i am optimizing a reactive distillation column. I have simulated a base case in Aspen Plus and now want to calculate its total annual cost in Matlab. while linking aspen Plus and Matlab i need...

01 October 2020 207 2 View

What happen if sebacic acid and glycerol were reacted for hour and then left the continued next day??

I have to end a reaction in process due to sone emergency. I was synthesizing poly glycerol sebacate. I reacted them at 170 under nitrogen for an hr and then stopped. Normal duration is 4 hr....

23 September 2020 7,827 5 View

Instead of gold or carbon coating, may I use platinum sputtering to get SEM image of a non-conducting material?

I actually don't have gold and carbon. I would like to know if I can do platinum sputtering on my sample to get SEM image? My sample is a non-conducting nanofiber

22 September 2020 2,470 3 View

How to check previous TC track record from the Japan Meteorological Agency (JMA)?

The TC track record can be checked easily from the Shenzhen Typhoon website, but how to check such records from the JMA website? Looking forward to a positive response.

31 August 2020 6,285 3 View

How to prevent hydrolysis of Pre-Polymer Poly Glycerol Sebacate?

I am working on encapsulating drug in Pre-PGS by solvent evaporation method. In which organic phase is PGS dissolved in THF, drug in water with tween 20. I am getting little particles at end. But...

02 April 2020 497 11 View

How to find Mean pixel intensity of ROI by using Image Processing Tool in MATLAB?

I am trying to find mean & SD of selected ROI via image processing tool. It is showing individual pixel intensity but not mean. Did I have to write a code for it?

25 February 2020 6,225 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View