There isn't universal agreement on what constitutes a sub-species, or even if this is a valid taxonomic level. As such, I doubt there is any kind of consensus on what degree of GC difference is typical of such divergence.

Further, what is the degree of variability within these populations? What kind of organism are we talking about here?

Hi Swetha

I agree with Mr. Lehmann, too. What kind of organism are we talking about here?

the first,  you have to determine significance of this 1% difference in the desired organism population.

Although I'm not sure a universal agreement on this issue

Hi Shaun G. Lehmann

Can you please elaborate on "what kind of organism we are talking about". Because, I have already stated that my organism was an algae. To be specific, Chara zeylanica.

And do you want the degree of variability in terms of the sequenced region?

Thank you